Water resources are important in large basins which are important places for human habitation and industrial and agricultural development. The background of editing this thematic issue was introduced and the general water resources situation and water quality status in four major large river basins in the Asian and African continents were briefly summarized to give readers general pictures of water resources development and management in these basins, and these large river basins are the Yellow River Basin, the Yangtze River Basin, the Indus Basin, and the Nile Basin. The thematic issue papers were classified into four clustered topical categories, and the main points of the papers in this thematic issue were summarized. Finally, the perspectives of future sustainable water resources development and management in large river basins were proposed.