Abstract:Objective: Evidence suggests that paeonifl orin may be involved in anticancer activities. Here, we have investigated the effects of paeonifl orin and correlative mechanisms on anti-invasion and anti-metastasis in human hepatocellular carcinoma (HCC) cell lines. Materials and methods:In the current study, we have applied 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay to determine the proliferative effect of HepG 2 and Bel-7402, two human hepatoma cell lines, and we have established a boyden chamber assay, a wound healing assay and cell adhesion assay to detect and quantify the invasion, metastasis and adhesion of both HepG 2 and Bel-7402. In addition, we have analyzed the protein expression of matrix metalloproteinas (MMP)-9, E-cadherin (E-cad) and extracellular signal-regulated kinase (ERK) in both cell lines through western blot analysis. Results: Paeonifl orin (6. 25-200 μM) had inhibitory effect on the growth of HepG 2 and Bel-7402 cell lines, and reduced signifi cantly invasion, metastasis and adhesion of HCC cell lines. In addition, paeonifl orin decreased the expression of MMP-9 and ERK in HepG 2 and Bel-7402 cells, and increased expression of E-cad in both cell lines. Conclusions: Paeonifl orin is effective anti-metastatic and anti-invasive agent for suppressing HCC invasion and metastasis (Fig. 5, Ref. 30). Text in PDF www. elis. sk.

Our results suggest a potential role for PSMB5 as a biomarker and therapeutic target for TNBC.

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Introduction: The purpose of our research was to evaluate MT1JP in breast cancer. Material and Methods: For clinical purpose, tissues were collected, and a correlation analysis ofMT1JP and miRNA-214 gene expressions was conducted. Using an in vitro study, MDA-MB-231 and MCF-7 cell lines were used as research objects in our research. Colony, flow cytometry, TUNEL, transwell, adhesion and wound healing assay were used to discuss the biological activities of the cells. In an in vivo study, tumor weight and volume were measured, and cell apoptosis was measured by TUNEL assay. The relative mechanism's proteins were evaluated by Western blotting or immunohistochemistry assay. Results: Compared with adjacent tissues, MT1JP and miRNA-214 gene expressions were significantly different (P<0.001, respectively). By in vitro and in vivo studies, the biological activities of the cells were significantly decreased in MDA-MB-231 and MCF-7 cell lines with MT1JP overexpression. The relative mechanism was correlated with miRNA-214/ RUNX3 axis. Conclusion: The overexpression of MT1JP suppresses the biological activities of breast cancer cells by regulation miRNA-214/RUNX3 axis in vitro and vivo study.