Penelitian ini bertujuan untuk mengetahui respon pembungaan tanaman Globba leucantha var bicolor Holttum terhadap volume pemberian air dan pupuk NPK Growmore. Penelitian dilakukan selama 3 bulan dari Bulan September hingga November 2020 pada rumah kasa artifisial berlokasi di Kecamatan Dayeuhluhur Kabupaten Cilacap Jawa Tengah. Metode yang digunakan Rancangan Acak Kelompok faktor tunggal sebanyak 12 perlakuan yang diulang 3 kali terdiri dari volume pemberian air dan pupuk NPK Growmore : 100% KAT tanpa pupuk; 100% KAT + pupuk 2 gram/ tanaman; 100% KAT + pupuk 4 gram/ tanaman; 80% KAT tanpa pupuk; 40% KAT + pupuk 2 gram/ tanaman; 80% KAT + pupuk 4 gram/ tanaman; 60% KAT tanpa pupuk; 60% KAT + pupuk 2 gram/ tanaman; 60% KAT+ pupuk 4 gram/ tanaman; 40% KAT tanpa pupuk; 40% KAT + pupuk 2 gram/ tanaman; 40% KAT + pupuk 4 gram/ tanaman. Hasil menunjukkan bahwa perlakuan volume pemberian air dan pupuk NPK tidak berpengaruh nyata terhadap panjang malai dan jumlah bunga per malai yang dihasilkan. Pemberian 100% KAT+ pupuk NPK 4 gram/tanaman menghasilkan panjang malai terbaik (10,43 cm) dan perlakuan pemberian air 40% KAT + pupuk 2 gram/tanaman menghasilkan jumlah bunga per malai terbanyak 24,0 bunga. Kata kunci : Air, Globba leucantha, Growmore, Pembungaan.
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