The increase consumption of natural substances has brought about in increasing demand on biological source, including Spirulina sp. This Spirulina sp. has been recognized to provide some natural substances such as natural colorant, source of vitamins, protein and some minerals. Different material state of Spirulina sp. in the form of fresh and dried form may cause different quantity and quality of natural substances in microalgae which posses a wide range of change including nutritional and natural substances. The aims of the study was to investigate the bioactive compounds resulted from different form of Spirulian sp (fresh and dried) quantitatively. The materials used were fresh and dried Spirulina sp which was extracted by ethanol. Analysis were subjected for phytochemical screening, flavoniod, phenolic acid and antioxidant activity (DPPH scavenging activity). The results showed that total flavonoid of fresh and dried are: 25.6615±1.62 and 110.1356±12.5 quercetin/gr extract ; phenolic acid : 2.117±0.99 and 6.92±0.03 GAE/gr extract; IC50 : 33.0755, respectively. Fresh sample of Spirulina platensis shows better results in bioactive compounds quantitatively and qualitatively compared to that of dried sample, and drying process at 40-50 o C does not give any significant different on nutritional quality of dried sample of Spirulina platensis compared to fresh samples.

Fish paste is popular condiment that possessing a characteristic appetite-stimulating aroma. Nowadays many fermented fish paste prefer to use lower salt to produce fish paste. Fermented fish paste from dried anchovy fish was prepared with 2% NaCl (w/w), fermented at room temperature for 8 and 32 days of fermentation. The dried anchovy fish obtain from Rejomulyo Market, Central Java, with + 7 cm of size. The method of research was experimental field. Biochemical changes during fermentation were investigated. Result revealed that during fermentation, pH and water content value were increased. Amino acid composition showed that the contents of glutamic acid, aspartic acid, lysine, leucin and arginin were higher than other amino acids. Free amino acid content of fermented fish paste was fluctuated during the fermentation period but most of free amino acids were increased. Almost all fatty acids decreased during fermentation except stearic acid, EPA and DHA. The essential fatty acid found was linolenic acid which was higher in initial period. Content of amino and fatty acids may contribute to the taste and flavor of fermented fish paste. From the nutritional view, the use of low salt concentration (2%) in producing fish paste was still able to contribute the fermentation process, avoid putrefaction and result in protein, some amino acid and fatty acid increase during early fermentation. But allegedly the shelf life of this paste is shorter because in the 32 days fermentation, the pH start to increase which means the volatile base compound formed already high.

Terasi merupakan produk fermentasi udang dengan penambahan garam. Fermentasi dengan garam menyebabkan perombakan protein menjadi asam amino misalnya asam glutamat sebagai penghasil cita rasa khas terasi. Kadar garam dan lama fermentasi merupakan faktor penting pada proses pembuatan terasi. Penelitian ini bertujuan untuk menganalisis pengaruh perbedaan konsentrasi garam dan lama fermentasi terhadap kualitas terasi rebon terutama kandungan asam glutamat. Bahan baku yang digunakan pada penelitian ini adalah rebon (Acetes sp.) kering tawar dengan panjang ±2 cm per ekor. Perlakuan garam dengan konsentrasi 2%, 8,5%, 15% dan lama fermentasi (8 hari dan 32 hari). Metode penelitian menggunakan metode experimental field dengan Rancangan Acak Kelompok (RAK) pola split plot in time 3 kali ulangan. Parameter yang diuji meliputi organoleptik, pH, kadar protein, asam glutamat, kadar air dan profil asam amino. Parameter kadar protein, asam glutamat dan profil asam amino pada fermentasi hari ke 32 yang diamati hanya perlakuan konsentrasi kadar garam terbaik pada hari ke 8. Nilai organoleptik terasi berkisar antara 7,65-8,32 artinya produk tersebut dapat diterima konsumen. Nilai pH Nilai pH terasi rebon berkisar antara 7,09 sampai 7,89. Konsentrasi garam 2% pada terasi rebon menghasilkan kadar protein dan asam glutamat tertinggi yaitu 47,14%+0,20 (dry base). Asam amino yang dominan pada terasi rebon adalah asam glutamat dan asam aspartat. Lama fermentasi menyebabkan penurunan asam glutamat terasi rebon.<br />Kata kunci: asam glutamat, fermentasi, garam, rebon

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