We provide a checklist of the herpetofaunal assemblage from Espora Hydroelectric Power Plant region (UHE Espora), southwestern of the state of Goiás, Brazil. Representatives of 32 amphibian and 71 reptile species were obtained during faunal monitoring and faunal rescue programs carried out in the study area. The obtained species list and distribution records are here discussed in an attempt to improve the still limited knowledge on Cerrado herpetofaunal assemblages.