Riparian buffers along streams can intercept eroding soils, contaminants, and nutrients, improving stream habitats and increasing the health of aquatic communities. Instream and riparian habitats and sh and benthic invertebrate communities were surveyed in a Minnesota stream draining an agricultural watershed before and after implementation of a state-mandated buffer law passed in 2014 and aimed at protecting water quality. Intensive habitat assessments, electro shing, and benthic invertebrate sampling were used at the same 13 sites in 2005 and 2018. Average buffer width nearly doubled between surveys, and instream abundance of ne sediments and embeddedness of coarse substrates by ne sediments both declined signi cantly within 1 to 3 years of buffer establishment. Stream sites also were signi cantly deeper with faster current velocities, and sites had increased ri e habitat and increased instream vegetative cover for sh after buffer mandates. However, sh and invertebrate biotic integrity scores, and other biotic community metrics, did not display signi cant improvements after buffer establishment. Stream habitats appear to respond quickly when intact and continuous riparian buffers insulate streams from surrounding agricultural activities, but improvements in biotic communities likely will require more time to adapt to changed habitat conditions.

Riparian buffers along streams can intercept eroding soils, contaminants, and nutrients, improving stream habitats and increasing the health of aquatic communities. Instream and riparian habitats and fish and benthic invertebrate communities were surveyed in a Minnesota stream draining an agricultural watershed before and after implementation of a state-mandated buffer law passed in 2014 and aimed at protecting water quality. Intensive habitat assessments, electrofishing, and benthic invertebrate sampling were used at the same 13 sites in 2005 and 2018. Average buffer width nearly doubled between surveys, and instream abundance of fine sediments and embeddedness of coarse substrates by fine sediments both declined significantly within 1 to 3 years of buffer establishment. Stream sites also were significantly deeper with faster current velocities, and sites had increased riffle habitat and increased instream vegetative cover for fish after buffer mandates. However, fish and invertebrate biotic integrity scores, and other biotic community metrics, did not display significant improvements after buffer establishment. Stream habitats appear to respond quickly when intact and continuous riparian buffers insulate streams from surrounding agricultural activities, but improvements in biotic communities likely will require more time to adapt to changed habitat conditions.