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Monoclonal antibodies and flow cytometry were used to study lymphocyte, monocyte and tumor cell antigen expression in uveal melanoma. Forty-one melanomas were studied after various forms of treatment. An antimelanoma antibody, 13A3E, stained 81.7% of the cells. Tumors treated with helium ions or ^l25I plaques had 13A3E staining reduced to 62.5%, p = 0.011. HLA-ABC stained 85.4%, and HLA-DR stained 7.0% of the cells. T cells (CD3 positive) comprised 4.5 % (range 0.1–29.2 %) of total cell population. Natural killer (NK) cells, B cells and macrophages were present in small numbers (mean < 2.5 % in each case). Tumors with numerous T cells ( > 5%), were used for T cell subtype studies. The mean helper/inducer (CD4) to cytotoxic/suppressor (CD8) ratio was 1.00 (range 0.11–3.15). CD8 decreased, and CD4 increased with age in unirradiated tumors (p < 0.02)

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