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A procedure was developed for preparing n-butyl N-trifluoroacetyl esters of amino acids for use in analysis by gas liquid chromatography. This derivative was found to have advantages over others investigated. Single chromatographic peaks were obtained for all of the common protein amino acids except tryptophan and arginine. Tryptophan gave two peaks but could be converted into a single derivative by longer acylation. Reproducibility of response was found to be good for derivatives carried through the entire chemical and chromatographic procedure, and no problems were indicated in the analysis of mixtures. Under anhydrous conditions the derivatives were stable with respect to time, and yield of derivative was found to be above 96% for each of six representative amino acids.

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