Serpophaga hypoleuca P.L. Sclater & Salvin, 1866, is a bird species distributed in northern South America, where it mainly inhabits shrubs on river islands and occasionally large river shores. Its distribution is not well known in Colombia where current records show the species present south of the Amazonas department and locally in Arauca and Vichada. We present a new locality in Colombia along the Guaviare River, in San José del Guaviare, which expands the known distribution of this species in Colombia, filling an information gap for the Guaviare region.