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<p>ABSTRACT</p><p><br />The objective of the experiment was to investigate the physical and chemical characteristic of eight genotypes of papaya i.e. IPB1, IPB 2A, IPB 3, IPB 3A, IPB 4, IPB 7, IPB 8, and IPB 9 on two stages of ripening period based on percentage of the yellow area of fruit peel (75% yellow and 100% yellow). The fruits were <br />picked at 25% yellow of fruit peel colour. The experiment was conducted in split plot wi th completely randomized block design. The main plot was ripening periods of 75% and 100% ripe, while the genotypes were taken at subplot. There was no significant different on physical and chemical characteristics between papaya at stadium 75% and 100% yellow. Flesh firmness of IPB 9 was better than IPB 1, IPB 4 and IPB 8. Ascorbic acid content of IPB 4 (107.36 mg/100 g) was higher than that of IPB 2A and IPB 3A. Carotenoid content of IPB 4 (29. 73 mg/100g) was higher than that of the other genotypes.</p><p><br />Key words: Carica papaya, physical characteristic, chemical characteristic, ascorbic acid, carotenoid</p>

Mangifera casturi Kosterm., a mango plant from Kalimantan Selatan, Indonesia, has limited genetic information, severely limiting the research on its genetic variation and phylogeny. We collected M. casturi’s genomic information using next-generation sequencing, developed microsatellite markers and performed Sanger sequencing for DNA barcoding analysis. These markers were used to confirm parental origin and genetic diversity of M. casturi hybrids. The clean reads of the Kasturi accession were assembled de novo, producing 259 872 scaffolds (N50 = 1 445 bp). Fourteen polymorphic microsatellite markers were developed from 11 040 microsatellite motif-containing sequences. In total, 58 alleles were produced with a mean of 4.14 alleles per locus. Microsatellite marker analysis revealed broad genetic variation in M. casturi. Phylogenetic analysis was performed using internal transcribed spacers (ITS), matK, rbcL, and trnH-psbA. The phylogenetic tree of chloroplast markers placed Kasturi, Cuban, Pelipisan, Pinari, and Hambawang in one group, with M. indica as the female ancestor. Meanwhile, the phylogenetic tree of ITS markers indicated several Mangifera species as ancestors of M. casturi. Thus, M. casturi very likely originated from the cross-hybridization of multiple ancestors. Furthermore, crossing the F1 hybrids of M. indica and M. quadrifida with other Mangifera spp. may have generated much genetic variation. The genetic information for M. casturi will be a resource for breeding improvement, and conservation studies.

The Environment is one of the factors influencing the development and productivity of the bee colony. Favourable environment will significantly support bee productivity. The factors which influence the bees activity include temperature, humidity, light intensity, wind speed, rainfall, and food availability. The aims of this study are to know the influence of environmental factors on activity and propolis production of stingless bees. This study used six colonies of Tetragonula laeviceps into two different locations, monoculture and polyculture nutmeg farm. The method used is a direct observation of the activities and propolis production of stingless bee and analyzed by t-test. The study results of T. laeviceps in monoculture and polyculture farms showed that the activity of the bees was influenced by environmental factors influencing propolis production. The activities would start when the temperatures ranged from 22-23 oC, humidity 70-88%, and light intensity 183-4344 lux. The peak activity of the bees occurred at the temperatures of 26-28 oC, humidity 55-71%, and light intensity 46.875-91.347 lux. The propolis produced by T. laeviceps was 27.79 g in the monoculture farm and 48.80 g in the polyculture farm. The significant difference was predominantly due to the environmental factors.

Rambai (Baccaurea motleyana) is one of the underutilized fruits native to Indonesia. Rambai has high antioxidant activities containing phenolic, flavonoid, and anthocyanin compounds, secondary metabolite compounds derived from sugar metabolism. The sugar metabolism involved several related genes. This research aimed to characterize genes putatively involved in sugar metabolism in Rambai. Six sugar gene families were identified from 37 077 contigs of the assembled-transcriptome database against to UniProt database using the BLASTX program. The six sugar-related genes were characterized involved nine contigs of sucrose-phosphate synthase (SPS), three contigs of sucrose-phosphatase (SPP), 14 contigs of sucrose synthase (SUS), 19 contigs of alkaline/neutral invertase (INV), one contig of cytosolic invertase (CINV) and five contigs of beta-fructofuranosidase (CWINV). This research aims to give a comprehensive study of the sugar metabolism mechanism in B. motleyana. The data also revealed the genes that encoded the enzymes that were putatively involved in sugar metabolism.