Objective: The purpose of this research is to explore the in vitro activities of white (Raphanus raphanistrum) and red (Raphanus sativus) radish root ethanol extract in decreasing cholesterol levels. Methods: Ultrasonification method was used in obtaining 96% ethanol extract of white and red radish root. The cholesterol levels were analyzed by visible spectrophotometry, which was validated using Lieberman-Buchard reagents. The decreased cholesterol levels were converted into IC50 values. Results: The results showed that the IC50 of 96% white and red radish root ethanol extracts were 743.7 and 634.7 µg/ml, respectively. The results of statistical analysis using the T-test obtained a significant value greater than the probability value (P) of 0.05. Conclusion: Therefore, it was concluded that the activities of 96% ethanol extract of white and red radish roots did not have a significant ability to reduce the in vitro cholesterol levels.