Urinary stones a worldwide problem for affecting more number of people. The urinary stones which formed to the deposition of few minerals & crystalline materials. Urinary stone samples are analyze by X-ray diffraction and electron microscopy. The present study have been to identify the minerals and elements, those are present in urinary stones and attempt to establish its morphological structure and elemental compositions. In the present study the minerals and morphological changes of different samples of human urinary stones are evaluated using XRD & SEM methods and the relationship with river water. The urinary samples are analyzed to identify the concentration of elements such as it Ca, Cu, Fe, K, Mg, Na, P, S and Zn by EDX method, also study some physical and chemical properties of river water and the relation with kidney stone formation.

The results of water examinations for Al-Najaf sea by used energy dispersive x-ray and laboratory examination and it compare with river water were pH value was 5.5, Chlorides 950 mg/l, Sulfate 834 mg/l and intractability of water was 811mg/l, for water samples from Al-Najaf sea. While were the results for the river samples of water in Al-Najaf city, it were pH value was 6.7, Chlorides 344 mg/l, Sulfate 290 mg/l and Intractability of water was 390 mg/l. The laboratory results showed the role of Trichoderma harzianum in reducing the clear pollution factors in the pollutant waters after detecting it with an electron microscope. Where the results of the biological treatment by Trichoderma harzianum showed that there clear significant differences between the initial tests for the pollutant waters of AL-Najaf sea and the sea waters that were treated as follows : pH value was 6.4, Chlorides 720 mg/l, Sulfate 400 mg/l and Intractability of water was 460 mg/l, for pollutant waters after treatment by Trichoderma harzianum.

This study was carried out to detect the contamination of the eyes with the black fungus and its presence in the eyes, where swabs were taken with the number of 50 swabs from the eyes, of twenty-five healthy people aged 15-30, and twenty-five elderly people aged 50-70 with diabetes. The results showed significant differences in the rate of isolation between healthy people and people suffering from chronic diseases, the results showed that percentage of isolation from elderly was 62.7 %, while the percentage of isolation from healthy people was 37.3%, also three other species were isolated (A.flavus, A.ochracus, A. terreus) in the rate of (16.9, 10.2, 3.4)% respectively in elderly people, but other species were isolated (A.flavus, A.ochracus ) in the rate of (11.9, 6.8)% respectively in healthy people.

The study aims to evaluate the use of rice and starch medium in preparing a culture medium for the development of some fungi isolated from the soil and compare it with standard medium PDA, where the fungi were identified microscopically. The media was prepared with the same components as the standard medium. The results showed that the rice and starch medium were significantly superior in the growth of fungi compared to the standard medium, as the rice and starch medium gave the average growth diameter of Aspergillus niger that reached 4.3-4.6 cm compared with the standard medium PDA which reached 4.1-3.8 cm. It was followed by Alternaria alternate at a rate of 3.6-3.4 cm compared to growth on standard medium 3.3-3.1cm, then Rhizopus stolonifera at a rate of 3.5-3.2 cm compared with growth on standard medium 3.2-3.1 cm, in growth on rice and starch culture medium, while it was less growth of Aspergillus flavus at a rate of 4.3-3.9cm compared with growth rates on the standard culture medium 4.4-4.2 cm.

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