Abtract. Chlorhexidine gluconate (CHX) has a bactericidal effect to Aggregatibacter actinomycetemcomitans (Aa) which causes periodontal disease. Therefore the controlled loading and release of CHX from multilayer thin film is beneficial for the local treatment of periodontitis. This study used ten bilayers of PDADMAC/PSSMA and PDADMAC/AL and loaded CHX into each film. Both un-loaded and loaded thin films were analysed using UV-Vis spectroscopy and AFM. The loaded film was thicker and rougher than the un-loaded film for both PDADMAC/PSSMA and PDADMAC/AL film. The loaded films were released in H2O2 for 24 h, and UV-Vis spectroscopy was measured and compared with the standard calibration curve of CHX. Results indicated that PDADMAC/PSSMA released 0.00052 M and PDADMAC/AL released 0.00071 M respectively. Both the loaded films were tested for toxicity to Aa. Neither film killed all colonies of Aa, however they reduced colony growth by 99% compared to the blank condition.