Fluorescent glass has advantages of homogeneous light emission, excellent thermal stability, and stable product quality as compared with traditional phosphors. Therefore, fluorescent glass in the field of LED has become a research hot spot in recent years. In this paper, Eu 3+ doped borate glasses were synthesized via the conventional melt-quenching technique. The luminescence properties and thermal stability of the fluorescent glass samples were characterized by fluorescence spectra. The doping concentration of RE 3+ ions is an important factor influencing the samples' luminescent performance. Based on the Van Uitert model, concentration quenching behavior for the prepared glass samples was studied. It is found that the fluorescence quenching of the samples is attributed to the nonradiative energy transfer through cross-relaxation mechanism between energy levels of Eu 3+ .As is known to all, the temperature dependence of fluorescence glass' luminescence is also important to the practical applications. The temperature quenching mechanism for the prepared glass samples was analyzed by Arrhenius model, and it was found that the temperature quenching could be ascribed to the crossover model. The activation energy for the prepared glass samples is obtained from fitting processes which is found to be (0.350.04) eV. The interaction between Eu 3+ ion and glass host was also analyzed. The result shows that the interaction between Eu 3+ ions and the glass host is weak coupling.