<em>Endophytic bacteria are a beneficial bacteria that live in plant tissue. Several studies have shown that certain endophytic bacteria can produce secondary metabolites that have health effects. The andaliman (Zanthoxylum acanthopodium DC) have several properties, one of which is as antibacterial against pathogenic bacteria. The most common bacterial infections in humans are caused by commensal bacteria in the body such as Escherichia coli and Staphylococcus aureus. This study used a qualitative data collection method with the type of experimental laboratory research to isolate the endophytic bacteria andaliman which had been tested against the pathogenic bacteria Escherichia coli and Staphylococcus aureus. The initial stage were isolation of endophytic bacteria and observation of colony characteristics, after obtaining isolates with different colony characteristics, a Gram stain test and a antagonist test for pathogenic bacteria were carried out. In this study, 252 colonies grew and there were 85 isolates with different colony characteristics. From all the isolates, there were two isolates that could inhibit the growth of Escherichia coli and seventeen isolates that could inhibit the growth of Staphylococcus aureus.</em>

Phyllosphere bacteria were bacteria that inhabit around the leaf surface. Several studies showed the potential of phyllosphere bacteria in producing bioactive compounds, one of which is antimicrobial compounds. Andaliman (Zanthoxylum acanthopodium DC.) was a plant from the Rutaceae family that was commonly found in North Sumatra, and its fruit was widely used as a spice in traditional cooking by the Batak tribe. Several studies showed that the andaliman terpenoid has antioxidant and antimicrobial activity and an immunostimulating effect. This study aimed to explore the potential of andaliman phyllosphere bacteria. The research design method was a qualitative experimental model. The research stages used in compiling this paper consisted of phyllosphere bacteria isolation, phyllosphere bacteria morphological characterization, and Gram stain. This study succeeded in isolating 206 colonies of andaliman phyllosphere bacteria with various colony forms and cell morphology. From 64 samples of phyllosphere bacterial isolate observed, we found 25 different colony forms, and all samples were Gram-positive bacteria. Microscopic observations of gram staining showed that 44 isolates (68.75%) were cocci, 17 isolates (26.56%) were in the form of bacillus, and three isolates (4.68%) were in the form of coccobacillus.

The incessant socialization of the government regarding health protocols to a new life order has resulted in most Indonesians becoming introspective in maintaining cleanliness. However, some of the community also experienced panic, so that they acted unnaturally by buying up more goods than they needed, including hand sanitizers. This is one of the reason the price of hand sanitizers has increased. That’s why people need to know how to make hand sanitizers so that they are no longer worry about the scarcity of hand sanitizers and can make their own hand sanitizers. The purpose of this activity is to meet the needs of residents for hand sanitizer as an antiseptic with WHO standards. The method used in training in making hand sanitizers in a direct demonstration. Demonstrations are also supported by a PowerPoint and video that is displayed repeatedly with the help of an LCD projector. This was done to repeat the key points of the demonstration sp that a good level of understanding was achieved by the citizens. Then the Citizen representativeswere asked to repeat the demonstration so that the activities of seeingm listening and practicing the knowledge  of making hand sanitizers were completed. Then at the end of the event, an evaluation was carried out using the discussion or question and answer method. The results of the training in making hand sanitizers have helped meet the needs of residents for hand sanitizers, helped residents to be more independent in meeting their needs for hand sanitizers, and increased knowledge and skills of residents regarding making hand sanitizers as WHO standard antiseptics