Green gram (Vignaradiata L.) is one of Indias most essential and widely cultivated pulse crops. Several biotic and abiotic factors cause the yield loss in green gram. Among the biotic factors, root rot is one of the devasting factors which can cause maximum yield loss under severe conditions. Ten pathogenic isolates of Macrophominaphaseolinacausing root rot in Greengramwere collected from different locations in the Perambalur District of Tamil Nadu. The isolates produced root rot symptoms under artificial inoculation to confirm the pathogenicity. Among these isolates, Macrophominaphaseolina 1 isolated from Valikandapuramwas virulent, which took for further study. To test the efficacy of fungal biocontrol agents against the Greengram root rot pathogen, ten Trichodermaviride were isolated from ten different places in the Perambalur District of Tamil Nadu and evaluated in vitro. Among the ten isolates tested against Macrophominaphaseolina, the isolate Tv- 1 isolated from the Esanai village of Perambalur District effectively arrested the mycelial growth of Macrophominaphaseolina, which recorded 61.00 per cent growth inhibition over control.

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