Long‐read sequencing technology is a powerful approach with application in various genetic and genomic research. Herein, we developed the pipeline for long amplicon high‐fidelity (HiFi) sequencing and then applied it for sequencing mitochondrial DNA (mtDNA) genomes from pools of 79 Tibetan Mastiffs. We amplified the mtDNA genome with long‐range PCR using two pairs of primers. Two rounds of circular consensus sequencing (CCS) were conducted and their accuracy was evaluated. The results indicate that the second round of CCS can improve the accuracy of HiFi reads. In addition, the analysis of 79 high‐quality mtDNA genomes shows the Tibetan Mastiffs from outside of the Tibetan Plateau experienced hybridization with other dogs. The high quality reads generator (HQGR) software is provided to facilitate data analyses, which is publicly accessible on GitHub (https://github.com/Caizf-script/HQGR). Our long amplicon HiFi sequencing pipeline can also be applied in various target enrichment strategies for small genomes and candidate genes.

In order to solve the problems of low recall rate and precision rate, high error rate, and long evaluation time in traditional evaluation methods, a comprehensive evaluation of economic management performance based on an improved fuzzy clustering algorithm is designed. The improved magnetic optimization algorithm was used to optimize the fuzzy C-mean algorithm, the improved fuzzy clustering algorithm was completed, and the improved fuzzy clustering algorithm was used to mine the economic management performance data. Using data mining findings and AHP’s weighting formula, a complete method for evaluating economic management effectiveness was developed. The BP neural network was improved using a genetic algorithm based on the index weight calculation findings, and the full-assessment model of economic management performance was constructed. Using this approach, it is possible to accurately and quickly assess the economic management performance of a company with a high rate of recall and accuracy; the error rate of a thorough assessment ranges between −3 percent and 4 percent; the average duration for an assessment is 0.81 seconds.