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Three treatment parameters of supercritical CO 2 have been optimized by a response surface method using central composite design to obtain isolate yield of flax rove. Influence of operating parameters including dosage of cellulase, temperature, and pressure on final response is evaluated. The results show that the experimental values are adequately fitted to a quadratic polynomial equation. The optimal isolate yield is obtained with 2% cellulase dosage under temperature of 50 °C and pressure of 20 MPa. Mathematical model is also applied to describe the kinetic behavior of the treatment process, revealing that supercritical CO 2 is a promising green solvent for the scouring and bleaching of flax rove.