Gypenosides III is a major bioactive component which is rich in Gynostemma pentaphyllum. For better utilization of the native resource, response surface methodology was used to optimize the extraction conditions of gypenosides III from G. pentaphyllum. The effects of three independent variables on the extraction yield of gypenosides III were investigated and the optimal conditions were evaluated by means of Box-Behnken design. The optimal conditions are as follows: ratio of ethanol to raw material 25, extraction temperature 58°C and ultrasonic time 25min. Under these conditions, the yield of gypenoside III is 1.216±0.05%, which is agreed closely with the predicted yield value.
With the continuous application and development of ITS, the nodes in VANET have more decision-making ability based on the enhancement of the computing power . It is known that TSF cares about the accurate Infrastructure-to-vehicle delivery. However, it requires all the intersections in the VANET have their stationary nodes, taking a lot of resources and thus making it difficult to be applied directly. This paper presents Data Forwarding based on Distributed-region for Multi-hop Infrastructure-to-vehicle data delivery ,which could guarantee the data delivery delay with the demanding probability, maximizing the effective use of the frequency resources and decreasing the delivery delay jitter in Infrastructure-to-vehicle delivery. The simulation result is consistent with the effectiveness and reliability of the algorithm.
This article aims to study the extraction conditions of the total flavonoids and lactones from the ginkgo biloba powder. First, the influencing factors and levels for the extraction were determined by the single factor method, and then the optimum extraction conditions were optimized by orthogonal experimental design. The results showed that the optimal conditions for ultrasonic extraction of the total flavonoids were as follows: 2mg cellulase and 0.1g raw material (ginkgo biloba powder) were dissolved in 10ml water solution. The pH of the solvent was 4.5 adjusted with hydrochloric acid. The materials were extracted in ultrasound bath at 55°C for 40min. The optimal conditions for ultrasonic extraction of the total lactones were as follows: 7mg cellulase and 0.1g raw material were dissolved in 10ml water solution with pH 4.9. The raw materials were extracted at 50°C for 35min. Under the optimum conditions, the peak area contents were nearly 90% and 58% for total flavonoids and lactones, respectively.
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