The elicitor Hrip1 isolated from necrotrophic fungus Alternaria tenuissima , could induce systemic acquired resistance in tobacco to enhance resistance to tobacco mosaic virus. In the present study, we found that the transgenic lines of Hrip1 -overexpression in wild type (WT) Arabidopsis thaliana were more resistant to Spodoptera exigua and were early bolting and flowering than the WT. A profiling of transcription assay using digital gene expression profiling was used for transgenic and WT Arabidopsis thaliana . Differentially expressed genes including 40 upregulated and three downregulated genes were identified. In transgenic lines of Hrip1- overexpression, three genes related to jasmonate (JA) biosynthesis were significantly upregulated, and the JA level was found to be higher than WT. Two GDSL family members ( GLIP1 and GLIP4 ) and pathogen-related gene, which participated in pathogen defense action, were upregulated in the transgenic line of Hrip1 -overexpression. Thus, Hrip1 is involved in affecting the flower bolting time and regulating endogenous JA biosynthesis and regulatory network to enhance resistance to insect.