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Abstract. For the increasingly demanding of real-time temperature monitoring in industrial and agricultural production, a data collection box based on wireless communication module NRF24L01 is designed, temperature is collected by a high-precision temperature sensor AD590.Design method of hardware and software of the system is described in detail, the configuration method of NRF24L01 is given. The application of this design in wireless temperature collection system is discussed.The experimental result shows that the design has realized two real-time monitoring on temperature of two points, it can display the value in different environment, a sounder is equipped in the design to alarm for over-temperature.