Amelogenin and its various derived peptides played important roles in promoting biomimetic mineralization of enamel. Previously, an amelogenin-derived peptide named QP5 was proved to be able to repair demineralized enamel....

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ObjectivesIncreasing evidence indicated circRNAs were involved in stem cells osteogenesis differentiation. Herein, we aimed to clarify the role of hsa‐circ‐0107593 during the osteogenesis process of human adipose‐derived stem cells (hADSCs) and the underlying mechanisms.MethodsThe ring structure of hsa‐circ‐0107593 was confirmed using RNase R treatment and Sanger sequencing. Nucleoplasmic separation and fluorescence in situ hybridization detected hsa‐circ‐0107593 distribution. Lentivirus and siRNA were used to modulate the expression of hsa‐circ‐0107593, and the binding relationship between hsa‐circ‐0107593 and miR‐20a‐5p was verified by luciferase assay and RNA immunoprecipitation. We detected the osteogenic activity of hADSCs through alkaline phosphatase staining, alizarin red S staining, real‐time polymerase chain reaction (RT‐PCR), western blot, and cellular immunofluorescence experiment. In vivo, micro‐computed tomography was performed to analyze bone formation around skull defect.ResultsRT‐PCR results exhibited that hsa‐circ‐0107593 was downregulated while miR‐20a‐5p was upregulated during hADSCs osteogenesis. In vivo and in vitro experiments results indicated that knocking down hsa‐circ‐0107593 promoted the osteogenic differentiation of hADSCs, while overexpression of hsa‐circ‐0107593 showed an inhibitory effect on hADSCs osteogenic differentiation. In vitro experiment results showed hsa‐circ‐0107593 acted as a hADSCs osteogenic differentiation negative factor for it inhibited the suppressing effect of miR‐20a‐5p on SMAD6.ConclusionKnocking down hsa‐circ‐0107593 acts as a positive factor of the osteogenic differentiation of hADSCs via miR‐20a‐5p/SMAD6 signaling.