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Entrepreneurial ecosystem has received increasing attention of scholars and practitioners in management. However, the key concept of symbiotic relationship promoting entrepreneurship ecosystem well-developed is not yet clear, including its constructing dimensions and antecedents. Based on an embedded exploratory case study of AliBaba’s entrepreneurial ecosystem, the paper develops the antecedents and dimensions of symbiotic relationship among the actors. We find that in the hub-based entrepreneurial ecosystem, the antecedents of symbiotic relationship are resource needs and opportunity perception, and its dimensions consist of mutual recognition, strategy fit, common goal and governance mechanism.