Enhanced monsoon duration and soil acidification from acid rain are expected to impact the distribution of microbial communities in surface and subsurface environments, although these impacts are poorly understood for most systems. In central China, soluble carbonate bedrock forms extensive karst landscapes. Current predictions are that the amount of monsoonal precipitation and acid rainfall in central China will increase, which is expected to lead to changes in the pH balance of karst ecosystems. To evaluate the role of pH, total organic carbon, and other geochemical parameters (e.g., Ca2+, Mg2+, NH4+, NOx, SO42-) in shaping bacterial communities within a single karst system in central China, samples were collected from the thin surface soils overlying Heshang Cave, cave sediments, and weathered cave passage rocks from the entrance, twilight, and dark zones, as well as from epikarstic drip waters inside the cave. Illumina sequencing of 16S rRNA genes and multivariate statistical analyses revealed that each tested community was distinct and the community variability was significantly correlated with pH, total organic carbon, and potassium concentrations. Specifically, surface soils were dominated by Acidobacteria, Verrucomicrobia and Planctomycetes, and diversity significantly decreased with acidic pH values. Nitrospirae, Gemmatimonadetes, Firmicutes, and Chloroflexi were unique to cave sediments, while Actinobacteria and Proteobacteria dominated weathered rocks and drip waters, respectively. The results reveal important implications regarding the effects of acidification on bacterial communities in karst areas, and on the control of pH in shaping bacterial communities throughout a karst system. Increased water flux into and through karst habitats due to monsoonal precipitation may result in deeper penetration of acidic solutions into karst and shift the bacterial communities inside the cave in the future.

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The origin of sedimentary dolomite is a subject of long-standing enigma that still awaits resolution. Previous studies have shown that room temperature synthesis of abiotic dolomite is rarely achieved and primary (proto-)dolomite precipitation is closely associated with microbial activities. In this study, we demonstrate through laboratory carbonation experiments that highly negative-charged clay minerals (as indicated by the values of zetal potential) such as illite and montmorillonitecan aid the precipitation of abiotic proto-dolomite under ambient conditions, whereas nearly-neutral charged kaolinite exerts negligible influence on such process. In comparison to montmorillonite, illite has higher surface-charge density, thus is more effective in catalyzing protodolomite precipitation. Furthermore, the signal of proto-dolomite in carbonate neoformations is enhanced with increasing concentrations of illite or montmorillonite. On the basis of these results, we suggest that clay minerals catalyze dolomite formation perhaps via electrostatic binding of Mg 2+ and Ca 2+ ions and simultaneously desolvating these strongly hydrated cations, a crucial step for dolomite crystallization. The resulting proto-dolomites display the morphologies, textures, and structures similar to those of biogenic dolomite reported before, which are considered precursors of ordered sedimentary dolomite. Therefore, our results offer a possible route to authigenic dolomite found in sedimentary environments.

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