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Abstract2-(Quinolin-2-yl)quinazolin-4(3H)-one (Q) was synthesized via the Brønsted acid-promoted tandem cyclization/dehydrogenation reaction with a good yield. Compound Q is a selective ‘turn-on’ fluorescent sensor for Zn2+ ion without interference by Cd2+. The 1:1 binding model of Q to Zn2+ was confirmed by the Benesi-Hildebrand analysis, Job’s plot analysis and a ultraviolet-visible (UV-Vis) titration experiment. Furthermore, the light-on fluorescent response can be observed by the naked eye under UV-lamp irradiation (365 nm).