Poly(ADP-ribosyl)ation (PARylation) is mainly catalysed by poly-ADP-ribose polymerase 1 (PARP1), whose role in gene transcription modulation has been well established. Here we show that, in response to LPS exposure, PARP1 interacts with the adenylateuridylate-rich element-binding protein embryonic lethal abnormal vision-like 1 (Elavl1)/human antigen R (HuR), resulting in its PARylation, primarily at site D226. PARP inhibition and the D226 mutation impair HuR's PARylation, nucleocytoplasmic shuttling and mRNA binding. Increases in mRNA level or stability of pro-inflammatory cytokines/chemokines are abolished by PARP1 ablation or inhibition, or blocked in D226A HuR-expressing cells. The present study demonstrates a mechanism to regulate gene expression at the post-transcriptional level, and suggests that blocking the interaction of PARP1 with HuR could be a strategy to treat inflammation-related diseases that involve increased mRNA stability.

Nature Communications 8 Article number:14632 (2017); Published: 8 March 2017; Updated: 31 March 2017 The original version of this Article contained an error in the spelling of post-transcriptional in the title of the paper. This has now been corrected in both the PDF and HTML versions of the Article.

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Seedlings ofMichelia macclurei,Cinnamomum camphora,RhodoleiachampioniiandMytilaria laosensiswere placed in open-top chambers (OTC) with three ozonic treatments including E20 (20 ppb), E40 (nature air, 40 ppb) and E160 (160 ppb) and two water treatments. Root biomass, stem biomass and leaf biomass total biomass and root/shoot ratio of four seedling types were evaluated. The results showed that there were no significant differences in impacts of ozone stress and drought stress on root, stem, leaf, and total biomass ofM. macclureiamong different treatments. The biomass ofM. laosensisdecreased with increasing ozone concentration, whereas biomass ofC. camphoraandR. championiichanged irregularly. Most of the biomass of four kind seedlings under ozone and drought intercross stresses was lower than those under ozone stress. The root/shoot ratios of four seedling species were all low. The ratios under high concentration ozone (160 ppb) were lower than the control treatment. And the ratios of different treatments under drought were lower than those were not under drought when they were under the same concentration of ozone.