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An amperometric ethanol biosensor was fabricated by integration of alcohol dehydrogenase (ADH) with meldolas blue (MB)/ordered mesoporous carbon (OMC) composite modified glassy carbon electrode (MB/OMC/GCE). The MB/OMC/GCE was highly sensitive for nicotinamide adenine dinucleotide (NADH) measurement (9.1 AE 0.25 mA/ mM) and gave a low detection limit of 0.21 AE 0.02 mM. The ethanol biosensor exhibited a wide linear range up to 6 mM with a lower detection limit of 19.1 AE 0.58 mM as well as a high sensitivity of 34.58 AE 2.43 nA/mM without suffering any interference from some common electroactive compounds.

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