The present investigation was conducted during Kharif season (2018-19) to study the interactive effect of biochar, FYM and nitrogen on uptake of nutrients, quality and yield of Blackgram at Research Farm, Department of Agronomy, Dr. PDKV, Akola. The experiment was laid out in Randomized Block Design with nine treatments and three replications by split plot design (RCBD). The  highest yield and yield attributing characters of blackgram were observed with soil application of biochar @ 10 t ha-1 + FYM @ 10 t ha-1 and nitrogen @ 20 kg ha-1. Nutrient content and uptake by blackgram was significantly highest in treatment where soil application of biochar @ 10 t ha-1 + FYM @ 10 t ha-1 and nitrogen @ 20 kg ha-1 was applied and it was on par with Biochar 5 t ha-1 + FYM 5 t ha-1 + Nitrogen 20 kg ha-1 and FYM 10 t ha-1 + Nitrogen 20 kg ha-1. The test weight was significantly highest in treatment receiving soil application of biochar @ 10 t ha-1 + FYM @ 10 t ha-1 and nitrogen @ 20 kg ha-1  and it was found to be on par with all other treatments expect treatment of control. Protein content of blackgram was increased (p<0.05) with the application of biochar @ 10 t ha-1 + FYM @ 10 t ha-1 and nitrogen @ 20 kg ha-1.