The seed borne myco-flora of cowpea cv. Phule Vithai was examined by blotter method, agar plate method, deep freezing, 2-4 D method and Test tube water agar seedling symptom test as recommended by ISTA. A total of four species of fungi viz. Alternaria alternata, Aspergillus flavus, Aspergillus niger, Fusarium oxysporum, Penicillium spp. and Fusarium moniliforme were reported. Amongst the methods used for detection of seed borne fungi, the standard blotter paper method is more effective followed by agar plate method, 2, 4-D method, deep freeze blotter paper method and test tube water agar seedling symptom test.
Background: Groundnut (Arachis hypogaea L.) is the fourth most important oil seed crop in the world. The early and late spots are a foliage disease more common and more destructive of groundnut which cause severe yield losses up to 70% and reduces the quality of the pod and fodder. Recent efforts have targeted on developing resistant source against leaf spot disease which are helpful for discarding the hazardous toxic chemical compounds used for controlling the disease. The current study aimed to identify the sources of resistance to leaf spot from the newly identified genotypes as well as from wild species and to study the way in which these were responding to the to the disease development.
Methods: Seventy three groundnut genotypes were screened against late leaf spot disease under artificial epiphytotic conditions in field at AICRP on Groundnut, MPKV, Rahuri during kharif 2015-16. The observations of disease intensity and rate of infection were recorded.
Result: Among the Seventy three groundnut genotypes, 10 genotypes showed resistant and 33 genotypes showed the moderately resistant reaction to leaf spot disease. The 25 entries showed the susceptible and 05 genotypes showed the highly susceptible reaction to the leaf spot disease. All the susceptible cultivars belong to A. hypogaea sub. sp. fastigiata var. fastigiata to as Spanish bunch. These 10 resistant genotypes of groundnut including two wild species viz., RHRG 6083, KDG 128, GPBD 4, ICGV 94118, ICGV 4983, ICG 12672, ICGV 13160 (A. batizocoi), ICGV 13165 (A. cardenasi), ICG 11426 and ICGV8193 were selected for intensive artificial screening under glasshouse conditions and also confirmed resistance. The resistant lines will be useful to develop introgression lines using marker-assisted backcrossing approach to improve foliar fungal disease resistance in popular groundnut varieties.
Agriculture's Cinderella' and 'Wonder crop of the 20 th Century' due to its qualities such as high protein (40%), good amount of carbohydrates (35 %), oil (20%) and ash (5%) content on oven dry basis. In India, soybean is mainly grown in the states of
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