Qualitative protein profile in the small intestine of singly and multiply infected mice were studied during ancylostomiasis. In singly infected animals, polypeptide changes occurred on day 4 and 9 of infection. In multiply infected animals also, the polypeptide pattern was markedly altered during day 4 and 9 of experiment. These studies demonstated that both low molecular and high molecular weight protein bands are formed due to antigen -antibody reactions in the small intestine of the host.

The present study was conducted to know the protein, DNA. RNA and amino acids level in the large intestine of male swiss albino mice infected orally each with 500 (group A), 1000 (group B) and 2000 (group C) larve of Ancylostoma caninum. All the mice of infected groups showed increased level of protein, DNA (except in group A), RNA and amino acids from day 1 to 30 of infection period compared to uninfected controls. It was understood that the infection induced significant alteration in the synthesis and/or release of these biochemical constitutents.