Glucose in blood is the most frequent analyzed parameter in a clinical chemistry laboratory. In Armed Forces Laboratories, copper reduction method (Modified Folin Wu) is commonly used. Here we have compared this method as well as O-Toluidine and GOD-POD method with reference UV-Hexokinase method. Both Modified Folin Wu and O-Toluidine showed upward deviation with substantial imprecision (CV=4.9% - 3.5% and 6% - 5.8% respectively) and inaccuracy (Average deviation = 5.76% and 10.68% respectively). GOD-POD method was found linear (up to 500 mg/dl), with good precision (CV=0.7% to 1.4%) and accuracy (Average deviation= -0.97%). This method is simple, rapid, economical and sensitive, and can be adopted to a routine colorimeter.

Lt Col VIPAN CHANDAR"', Brig CH GIDVANI + Dr AK GUPTA#, Lt Col CG WILSON"'''' Lt Col YV SHARMA++ ABSTRACf One hundred and fifty healthy children in the age group of 0-12 years were studied to establish normal levels of lipid profile. They were divided into six equal groups viz., newborns, 0-1 year, 1-4 years, 4-7 years, 7-10 years and 10-12 years. The mean values ± SD for total cholesterol, lIDL cholesterol, WL cholesterol and triglycerides were 70 ± 14.48 mg/dl, 35.1 ± 8.04 mg/dl, 23.8 ± 10.62 mg/dl and 50.9 ± 20.77 mg/dl respectively for male neonates and 71.8 ± 13.96 mg/dl, 34.6 ± 6.55 mg/dl, 25.5 ± 9.29 mg/dl and 57.2 ± 18.57 mg/dl respectively for female neonates. These values increased to 158.7 ± 21.23 mg/dl, 60.7 ± 11.70 mg/dl, 80.3 ± 19.26 mg/dl, 86.7 ± 28.80 mg/dl in males and 161.6 ± 23.09 mg/dI66.7 ± 8.75 mg/dl, 75.8 ± 20.26 mg/dl and 93.2 ± 44.09 mg/dl in females respectively between 10-12 years of age, The various lipid fractions were at the lowest level at birth and increased significantly during first year of life, there after showing a much slower rise in levels. There was no significant difference in values between male and female children. M]AFI : 1994 : 50 : 101-104

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Albumin excretion in microalbuminuria range is one of the earliest manifestation of nephropathy, specially in diabetes mellitus. The modified dye binding method using bromo-phenol blue was studied in 27 healthy controls and 54 patients of diabetes mellitus, negative for albuminuria by albustix test. The analytical recovery (99.4 to 104.0%), within run coefficient of variation (0.8 to 0.36%) and day-to-day coefficient of variation (2.39 to 0.82%), for low and higher range were within acceptable limits. The values in controls ranged as follows: urinary albumin concentration (mg/L) 7.7 - 28.4 in 2-hour specimen and 10.3 - 29.2 in overnight specimen; albumin excretion rate (µg/min) 7.8 - 29.7 in 2-hour and 9.2 - 29.6 in overnight specimen; and albumin creatinine ratio (mg/g) 10.6 - 29.6 in 2-hour and 11.9 - 29.6 in overnight specimens. Correlation analysis of various albumin excretion parameters revealed excellent correlation between estimations from overnight and 2-hour samples for albumin-creatinine ratio (r = 1.00) and albumin excretion rate (r=0.96). Equally good correlation was observed between 2-hour albumin-creatinine ratio and albumin excretion rate (r=0.95). In 10 of 54 patients excretion rate was more than 200 µg/min and could have been detected by repeat albustix test. Of the 36 positive for microalbuminuria, 21 had one or more target organ involvement. There was no target organ involvement in 8 patients negative for microalbuminuria. Screening for microalbuminuria by this simple and economic method, using 2-hour albumin-excretion rate or albumin-creatinine ratio could be one of the earliest investigations in diabetic patients.

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