The objective of this study was to evaluate bull sperm kinematics after centrifugation through a single layer of a colloid [Single Layer Centrifugation (SLC)]. Ejaculates from 20 bulls were extended and stored at 4-6°C for 24 h during transport to the laboratory for SLC through Androcoll-B, followed by measurement of sperm kinematics in all samples. Total motility (86% and 88% for uncentrifuged and SLC samples, respectively) and progressive motility (84% for both the groups) were similar (p > 0.05). In contrast, straightness (STR) (0.65 vs 0.69), linearity (LIN) (0.32 vs 0.35) and beat cross frequency (BCF) (22.3 vs 23.6 Hz) were significantly higher in the SLC-selected samples than in the uncentrifuged samples, whereas velocity of the average path (VAP) (95 vs 90 μm/s), curvilinear velocity (VCL) (192 vs 180 μm/s), amplitude of lateral head deviation (ALH) (7 μm vs 6.5 μm) and hypermotility (49% vs 38%) were significantly decreased. The kinematics of the samples with the poorest motility was improved most by SLC. In conclusion, even though SLC had no direct effect on total and progressive motility, it appeared to have a positive influence on several other kinematic parameters that may be important for fertilization after artificial insemination.

Spotted buffalo, an exotic species that exists in Tana Toraja, South Sulawesi, Indonesia, is getting extinct due to high number of slaughtered during a funeral ceremony, called Rambu Solo', as well as special treatments that do not allow the male spotted buffaloes perform natural mating activity. According to that, the research was trying to start conservation program by collected the cauda epididymal sperm soon after slaughtered. Two egg yolk-based extenders with different buffers, tris hydroxyl amino methane (TEY20) and trisodium citrate dehydrate (CEY20), were used as comparison to evaluate the post-thawed epididymal sperm quality and fertilizing capacity in artificial insemination program. The results showed that the post-thawed progressive motility of epididymal sperm was 40% and 39.17%, while viability was 65.99% and 63.26% and membrane integrity was 65.43% and 63.03% in TEY20 and CEY20 extenders, respectively. The success rate of pregnancy was 46.67% using post-thawed epididymal sperm in TEY20 and 40% using the one in CEY20 extenders. In conclusion, tris-based and citrate-based extenders have similar ability to maintain the epididymal sperm quality and its fertilizing capacity. Key words: artificial insemination, epididymal sperm, spotted buffalo ABSTRAKKerbau belang merupakan jenis kerbau unik yang ditemukan di Tana Toraja, Sulawesi Selatan. Populasi ternak ini menurun secara drastis karena tingginya laju pemotongan pada upacara adat Rambu Solo', serta keyakinan dalam adat yang tidak memperbolehkan kerbau belang jantan melakukan aktivitas reproduksi. Berdasarkan hal tersebut, studi ini dilakukan sebagai langkah awal proses konservasi dengan menggunakan cauda epididimis sebagai sumber sperma potensial. Studi ini menggunakan dua jenis bufer dalam bahan pengencer berbasis kuning telur, yaitu tris hidroxil amino metana (TEY20) dan trisodium sitrat dehidrat (CEY20), untuk menguji kualitas dan daya fertilitas sperma pasca-thawing dalam proses inseminasi buatan. Hasil yang diperoleh menunjukkan bahwa persentase motilitas progresif adalah sebesar 40% dan 39.17%, dengan persentase hidup mati sebesar 65.99% dan 63.26%, serta persentase membran plasma utuh sebesar 65.43% dan 63.03% dalam bahan pengencer TEY20 dan CEY20 secara berturut-turut. Dapat disimpulkan bahwa bahan pengencer berbasis tris dan sitrat memiliki kemampuan yang sama dalam mempertahankan kualitas dan daya fertilitas sperma epididimis kerbau belang pasca-thawing.

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