A novel biosensor was developed based on the cation-exchange of CuS nanoparticles (NPs) and Cu(i)-based click chemistry of functionalized gold nanoparticles (AuNPs). As a proof-of-principle, novel applications of this method as a versatile biosensor for single-nucleotide polymorphisms (SNPs) and DNA methyltransferase (MTase) were presented.

Thermoplastic vulcanisates (TPVs) based on ethylene-vinyl acetate copolymer (EVA)/styrenebutadiene rubber (SBR) blends were prepared by dynamic vulcanisation, with the TPVs being reinforced by carbon black (CB). Experimental results indicated that the mechanical properties of dynamically vulcanised EVA/SBR blends were enhanced remarkably by the incorporation of CB. Morphology study showed that the SBR particles with average diameter of 20 μm were dispersed evenly on the etched surface of EVA/SBR/CB TPVs. The Mullins effect could be observed in the stress-strain curves of EVA/SBR TPVs and EVA/SBR/CB TPVs during the uniaxial loading-unloading cycles. Compared with EVA/SBR TPVs, CB reinforced EVA/SBR TPVs had the relatively higher stress, residual deformation and internal friction loss.