We developed a protocol for preparing cardiac cell culture from rat heart enriched with regional stem cells based on clonogenic properties and proliferation in culture in a medium with low serum content. Experiments on WAG rats with experimental ischemic myocardial damage showed that implantation of autologous regional stem cells into the left ventricle reduced the volume of cicatricial tissue, promoted angiogenesis in the damaged zone, and prevented the risk of heart failure development.

Detergents are effective decellularization agents in order to obtain cell-free matrixes. However, the toxicity of residual detergent prevents the adhesion of cells to the matrix and the realization of their functions. In this study, the degree of influence of residual detergents on mesenchymal stromal cells after a series of washes was investigated. The human pulmonary valve were treated in three ways: 1% solution of sodium dodecyl sulfate, 1% solution of sodium deoxycholate and a combination of these solutions with the reduction of the concentration to 0.5%. After each of 8 steps washing buffer were collected. We investigated influence of residual detergents on cytotoxicity and effect on the metabolic activity of MSCs. Histologic analysis showed sufficient tissue decellularization in all groups. Matrixes were washed by successive incubations with 200 ml of phosphate buffer. Detergent concentrations in all samples ceased to be toxic only to the 4-cycle wash, and had no effect on the metabolism and viability of human MSCs. After 4-8 washing cycles decellularized pulmonary trunk fragments were successfully settled by mesenchymal stromal cells. Thus, a few successive washing fabrics pulmonary valve after decellularization reduce residual detergent to a safe level and lead to successful repopulation of acellular scaffolds with MSCs.

The results of the effect of immunomodulating biologically active drugs on the immune response of laboratory animals are presented. To study the processes, a model of experimental Rauscher leukemia was used in mice of pure lines. The morphological changes in the peripheral blood of Bagg Albino C (BALB / c) mice with experimental Rauscher's leukemia after the use of mononuclear stem cell and Subalin preparations were studied. Groups of animals were formed for the experiment: control and three experimental (infected). The animals of the experimental groups were examined simultaneously by intraperitoneal injection of the appropriate biomaterial. It was found that the use of mononuclear stem cells and Subalin does not prolong the life of infected animals. During the observation period, all infected mice died within 11 months. After infection with Rauscher leukemia, a quantitative and qualitative change in blood cells occurs. A shift to the left was noted in the leukogram, eosinophilia and monocytosis were revealed. In the process of experimental reproduction of the chronic form of the disease, the size of the spleen of mice was estimated. An increase in the spleen was observed in the experimental group of animals infected with the Rauscher leukemia virus on the 4th week after infection. It was found that Subalin has a pronounced effect on the leveling of hematological parameters and leukopoiesis in infected mice. In the group where stem cells were used together with the antigen, a slight decrease in the number of lymphocytes and an increase in stab and segmented neutrophils were recorded. In the experimental groups with the use of mononuclear stem cells and Subalin, an increase in young cells in the leukogram was noted.

To maintain a healthy immune system in animals, especially under conditions unfavorable for viral leukemia, a promising delivery system of biologically active molecules is considered – Subalin, a live delivery system. As a result of research, it has been established that with intraperitoneal and intravenous administration to animals, Subalin is harmless to animals, even at doses many times higher than the therapeutic one. With the introduction of the drug Subalin in animals stimulated humoral and cellular immunity, as evidenced by morphofunctional reorganization of the lymphoid system (spleen, lymph nodes). With the simultaneous administration of Subaline with a virus-containing suspension, subtility therapy had a stabilizing effect on the macroorganism, restraining the development of viral carcinogenesis.