Immobilization of the bacterium Agrobacterium tumefaciens UP-3, which was isolated from soil and sewage in the Shengli Oilfield, was studied in this paper. The suitable culture condition of growth cells was determined. The effects of immobilization conditions and materials on the desulfurization performance of immobilized cells were investigated. The results showed that the immobilized cells with mixture of sodium alginate (SA) and polyvinyl alcohol (PVA) as the immobilized carrier had good biodesulfurization characteristics; The optimum operation immobilization conditions are 4° C, the concentration of SA being 3% (w), the concentration of PVA and SA being 7% (w), and the ratio of carriers (ml) and cells (g) being 20. When DBT addition is 2.7 mM, the DBT degradation rate of immbobilized cells is above 60%.

The activated carbon was prepared from the Jing Bo shot coke using KOH as the activation agent by microwave heating. The effect of microwave radiation time, the microwave power and the ratio of activation to shot coke on the properties of the activated carbon were investigated in detail. The result showed that the optimized condition of preparation of activated carbon is as followed: the ratio of activation agent to shot coke is 3:1, the microwave power 900 W and the microwave radiation time is 40 min. In this condition, the BET surface area of the activated carbon is 2668 m2/g; the iodine adsorption value 2281 mg/g and the benzene adsorption value 1368 mg/g.