A pot experiment was conducted at Banaras Hindu University, Varanasi (U.P.) to assess the interactive effect of nitrogen (N) and sulphur (S) on product and nutrient content of mustard. Results showed that the seed yield increased significantly with increasing nitrogen level upto 100 kg N. Further increase in N level i.e. 150 kg N ha-1 had no significant effect on seed yield  attributing characters. Number of seeds siliqua-1, 1000 seed weight, straw yields, nutrient (N, P and K) uptake and available nutrients increased significantly with 100 kg N and 25 kg S ha-1over other treatments. Con­sidering the combined effect of N and S, and their ratio in plant the treatment combination 100 kg N and 25 kg S ha-1 produced the maximum significant results.