The aims of this study are to isolate thermotolerant yeasts and evaluate their fermentation capacity for the production of three-leaf cayratia (Cayratia trifolia L.) wine. Materials and methods Culture and materials Fifty-three samples of Cayratia trifolia were collected from 13 provinces in the Mekong Delta region. This was carried out in three phases: I: the C. trifolia berries were collected from the four provinces of Kien Giang, An Giang, Dong Thap, and Long An.