COX-2 induces the proliferation and invasion of oral squamous cell carcinoma. In the present study, the role of the COX-2 gene in the tongue cancer cell proliferation and invasion was investigated. A short hairpin RNA (shRNA)method was used to knock down COX-2 gene expression and investigate the relationship between COX-2 and VEGF-C, and the role of the COX-2 gene for proliferation and invasion was also investigated in the tongue cancer cell Tca8113. COX-2 gene overexpressed in tongue cancer cell line. Suppressing the expression of COX-2 by shRNA could decrease cell proliferation comparing with control shRNA. Nevertheless, depressing COX-2 gene expression by shRNA reduced VEGF-C expression on both mRNA and protein levels. VEGF-C gene expression could be regulated by COX-2 gene. Our results suggested that COX-2 played essential roles in the proliferation and metastasis of tongue cancer, and COX-2 could serve as a potential chemotherapy target for tongue cancer.

In this preparation, FCCU main fractionator and corresponding absorption-stabilization system are systemically simulated on the basis of industrial data. Through analysis, a heat exchanging column is added to improve the flowsheet before rich gas cooler to make good use of heat of the compressed rich gas. To decrease LPG lost along with dry gas, a dry gas compression-expansion refrigeration circle is proposed to offer refrigeration capacity in place of the cooling water for the absorber pumparounds. Compared with the regular scheme, energy consumption and C3+ components in dry gas is reduced respectively by 17.18% and 7.16% and LPG yield is increased by 0.41% simultaneously.