No abstract

A novel separating and detecting method of urinary proteins by non-gel sieving electrophoresis-free flow electrophoresis on the microfluidic chip was proposed in this article. Human serum albumin and human transferrin labeled by fluorescein isothiocyanate were mixed and taken as the synthesized urinary protein samples. Factors affected on separation, such as the rate of electric fields applied in two dimension, the addition in the buffer, the concentration, and pH of buffer, were optimized, that is, 10% (w/v) dextran, 0.5% ethanediamine, 0.2 mol L 21 Tris-boric acid, pH 9.3, the ratio of the applied electric field in two dimensions 1:4.8, respectively. Fluorescein isothiocyanate-human serum albumin and fluorescein isothiocyanate-transferrin were efficiently separated in 2 min, and the resolution of two proteins achieved was 2.2 by the designed microfluidic chip. Then, the urine samples of nephropathy patient were analyzed by the proposed nongel sieving electrophoresis-free flow electrophoresis on the microfluidic chip. The results matched well with those detected by common clinical methods.

Operating temperature is a key factor affecting the output power of a crystalline silicon solar cell (c-Si SC). Based on solving basic semiconductor equations, Maxwell equations and heat flow equations by finite difference method, this work has theoretically investigated the influences of microfluidic cooling system on the temperature distributions and output parameters of an industrial c-Si SC packaged as a module form, where the cooling system is installed the interface between the aluminum electrode and back ethylene vinyl acetate layer and the cooling medium flowing through microchannel is selected as water. Under the influences of the cooling system, the back surface temperature of the c-Si SC is fixed at about water temperature and the front surface temperature will decrease with the water temperature decreasing. The temperature distributions of c-Si active layer and aluminum layer decrease approximately linearly along the two layer’s thickness directions. The cooling effects of the cooling system are mainly determined by water temperature and when the filling factor of microchannel is larger than 0.1, its influences on the temperature distribution of the c-Si SC are small. In the environmental temperature range from -15℃ to 60℃, the increase rates of Voc, FF and η per degree Celsius with the water temperature decreasing are about 0.0014V, 0.036% and 0.05%, respectively.