The pre-metastatic niche educated by primary tumor-derived elements contributes to cancer metastasis. However, the role of host stromal cells in metastatic niche formation and organ-specific metastatic tropism is not clearly defined. Here, we demonstrate that lung epithelial cells are critical for initiating neutrophil recruitment and lung metastatic niche formation by sensing tumor exosomal RNAs via Toll-like receptor 3 (TLR3). TLR3-deficient mice show reduced lung metastasis in the spontaneous metastatic models. Mechanistically, primary tumor-derived exosomal RNAs, which are enriched in small nuclear RNAs, activate TLR3 in lung epithelial cells, consequently inducing chemokine secretion in the lung and promoting neutrophil recruitment. Identification of metastatic axis of tumor exosomal RNAs and host lung epithelial cell TLR3 activation provides potential targets to control cancer metastasis to the lung.

An effective structural doping approach has been described to modify the photoelectrochemical properties of g-C 3 N 4 by doping with nonmetal (sulfur or phosphorus) impurities. Here, the substitutional and interstitial doped models of g-C 3 N 4 systems were constructed with different doped sites, and then their dopant formation energies and electronic properties were performed to study the stability and visible-light photoactivity using firstprinciples density functional theory, respectively. Our results have identified that an S atom preferentially substitutes for the edge N atom of g-C 3 N 4 ; however, a P atom preferentially situates the interstitial sites of in-planar of g-C 3 N 4 . Furthermore, it is demonstrated that the doping with nonmetal impurities reduces the energy gap to enhance the visible-light absorption of g-C 3 N 4 . The increased dispersion of the contour distribution of the HOMO and LUMO brought by doping facilitates the enhancement of the carrier mobility, while the noncoplanar HOMO and LUMO favor the separation of photogenerated electron−hole pairs. Especially, P interstitial doping shows a prominent potential due to the appearance of a new channel for carrier migration. It should be pointed out that the proper doping form should be controlled, so that reasonable photoelectrochemical properties can be achieved.

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Objectives There is conflicting evidence on the efficacy of Traditional Chinese Acupuncture (TCA), and the role of placebo effects elicited by acupuncturists’ behavior has not been elucidated. We conducted a 3-month randomized clinical trial in patients with knee osteoarthritis to compare the efficacy of TCA to sham acupuncture, and examine the effects of acupuncturists’ communication style. Methods Acupuncturists were trained to interact in one of two communication styles: ‘high’ or ‘neutral’ expectations. Patients were randomized to one of 3 groups: waiting list, ‘high’ or ‘neutral’, and nested within style, TCA or sham acupuncture over 6 weeks. Sham acupuncture was performed in non-meridian points, with shallow needles and minimal stimulation. Primary outcome measures were: Joint-specific Multidimensional Assessment of Pain (J-MAP), Western Ontario McMaster Osteoarthritis Index (WOMAC), and satisfaction. Results 455 patients who received treatment (TCA or sham) and 72 controls were included. No statistically significant differences were observed between TCA or sham acupuncture, but both groups had significant reductions in J-MAP and WOMAC pain compared to the waiting group (-1.1, -1.0, and -0.1, p<0.001; -13.7, -14, -1.7, p<0.001). Statistically significant differences were observed in J-MAP pain reduction and satisfaction, favoring the ‘high’ expectations group. Fifty-two percent and 43% in the TCA and sham groups thought they had received TCA (kappa=0.05), suggesting successful blinding. Conclusion TCA was not superior to sham acupuncture. However, acupuncturists’ style had significant effects on pain reduction and satisfaction, suggesting that the analgesic benefits of acupuncture can be partially mediated through placebo effects related to the acupuncturist's behavior.

Global polarization of Λ hyperons has been measured to be of the order of a few tenths of a percent in Au+Au collisions at √ s N N = 200 GeV, with no significant difference between Λ andΛ.These new results reveal the collision energy dependence of the global polarization together with the results previously observed at √ s N N = 7.7 -62.4 GeV and indicate noticeable vorticity of the medium created in non-central heavy-ion collisions at the highest RHIC collision energy. The signal is in rough quantitative agreement with the theoretical predictions from a hydrodynamic model and from the AMPT (A Multi-Phase Transport) model. The polarization is larger in more peripheral collisions, and depends weakly on the hyperon's transverse momentum and pseudorapidity η H within |η H | < 1. An indication of the polarization dependence on the event-by-event charge asymmetry 3 is observed at the 2σ level, suggesting a possible contribution to the polarization from the axial current induced by the initial magnetic field. PACS numbers: 25.75.-q, 25.75.Ld