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Auxin response factors (ARFs), member of the plant-specific B3 DNA binding superfamily, target specifically to auxin response elements (AuxREs) in promoters of primary auxin-responsive genes and heterodimerize with Aux/IAA proteins in auxin signaling transduction cascade. In previous research, we have isolated and characterized maize Aux/IAA genes in whole-genome scale. Here, we report the comprehensive analysis of ARF genes in maize. A total of 36 ARF genes were identified and validated from the B73 maize genome through an iterative strategy. Thirty-six maize ARF genes are distributed in all maize chromosomes except chromosome 7. Maize ARF genes expansion is mainly due to recent segmental duplications. Maize ARF proteins share one B3 DNA binding domain which consists of seven-stranded β sheets and two short α helixes. Twelve maize ARFs with glutamine-rich middle regions could be as activators in modulating expression of auxin-responsive genes. Eleven maize ARF proteins are lack of homo- and heterodimerization domains. Putative cis-elements involved in phytohormones and light signaling responses, biotic and abiotic stress adaption locate in promoters of maize ARF genes. Expression patterns vary greatly between clades and sister pairs of maize ARF genes. The B3 DNA binding and auxin response factor domains of maize ARF proteins are primarily subjected to negative selection during selective sweep. The mixed selective forces drive the diversification and evolution of genomic regions outside of B3 and ARF domains. Additionally, the dicot-specific proliferation of ARF genes was detected. Comparative genomics analysis indicated that maize, sorghum and rice duplicate chromosomal blocks containing ARF homologs are highly syntenic. This study provides insights into the distribution, phylogeny and evolution of ARF gene family.

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Abstract-With the increase of low power devices, the design of a compact and efficient rectenna is essential for supplying energy to the devices. This paper presents a compact rectenna for high efficient WiFi energy harvesting. A novel fractal geometry is introduced in the design of antenna for miniaturization, and the ability to harvest WiFi energy is enhanced due to its characteristics of self-similarity and space filling. Besides, a single stub matching network is designed to achieve high conversion efficiency with a relatively low input power ranging from −20 dBm to 0 dBm. Simulation and experiments have been carried out. The results show that the proposed antenna features a good characteristic of reflection coefficient and realized gain at WiFi band. The highest RF to DC conversion efficiency of the rectenna is up to 52% at 2.45 GHz with the input power of 0 dBm. This study demonstrates that the proposed rectenna can be applied to a range of low power electronic applications.

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