The herbicide propisochlor can be introduced into the water cycle via agricultural activities, thus possibly leading to harmful ecological effects. It is significant for the development of a rapid method for propisochlor analysis in water. In this work, a rapid homogeneous immunoassay based on fluorescence polarization immunoassay (FPIA) was firstly developed for the determination of propisochlor in pond water. The developed FPIA assay can be completed within only 14 min; the 50% inhibition concentration (IC 50 ) was 125.1 ng/mL and the limit of detection was 8.4 ng/mL. The recoveries of propisochlor in pond water ranged from 80.0% to 86.6%. A high performance liquid chromatography confirmation showed a good correlation with the developed FPIA (R 2 = 0.9748). The developed FPIA exhibited satisfactory rapidity, high sensitivity and excellent specificity, meeting the standards of the screening assay of propisochlor in pond water on site. The molecular modeling showed that the alkoxyalkyl of chloroacetamide herbicides exerted important effects on the herbicide-antibody binding.

Macadamia oil cake (MOC) is a type of macadamia nut by‐product, that is extremely rich in amino acids and has beneficial health effects. It lowers blood lipid levels and regulates the intestinal microbiota. MOC effectively attenuated total cholesterol (TC), triglyceride (TG), low‐density lipoprotein cholesterol (LDL‐C), and high‐density lipoprotein cholesterol (HDL‐C) levels in model rats. Depending on the morphology of the colon, MOC can effectively attenuate damage to the tissue structure. The 16S rDNA gene of the rat intestinal microbiota was sequenced using Illumina PE250 high‐throughput sequencing technology, and the changes in the intestinal microbiota in each group are discussed. Supplementing MOC at different doses significantly increased the microbiota of Dorea, Erysipelotrichaceae, Stercoris, etc. in the intestinal tracts of rats fed a high‐fat diet. Therefore, MOC can be included in lipid healthy dietary patterns to lower lipid characteristics and restructure the intestinal microbiota. Future clinical trials are required to determine the therapeutic effects and mechanisms of hypolipidemia.