A deep eutectic solvent (choline chloride (ChCl)-urea) was chosen to extract flavonoids from
Moringa oleifera
leaves (F
MO
L), the condition of extraction was tailor-made, under the optimal extraction conditions (material-to-liquid ratio of 1:60 g/mL, extraction time of 80 min, extraction temperature of 80 °C), the highest extraction efficiency reached 63.2 ± 0.3 mg R/g DW, and nine flavonoids were identified. Then, the biological activities including antioxidant activities, antibacterial activities, and anti-tumor activities were systematically studied. F
MO
L was superior to positive drugs in terms of antioxidant activity. As to DPPH investigation, the IC
50
of F
MO
L and Vc were 64.1 ± 0.7 and 176.1 ± 2.0
µ
g/mL; for the ABTS, the IC
50
of F
MO
L and Vc were 9.5 ± 0.3 and 38.2 ± 1.2
µ
g/mL, the FRAP value of F
MO
L and Vc were 15.5 ± 0.6 and 10.2 ± 0.4 mg TE/g, and ORAC value of F
MO
L and Vc were 4687.2 ± 102.8 and 3881.6 ± 98.6
µ
mol TE/g. The bacteriostatic (MICs were ≤ 1.25 mg/mL) activities of F
MO
L were much better than propyl p-hydroxybenzoate. Meanwhile, F
MO
L had comparable inhibitory activity with genistein on tumor cells, IC50 was 307.8
µ
g/mL, and could effectively induce apoptosis in HCT116. Microcapsules were prepared with xylose-modified soybean protein isolate and gelatin as wall materials; after that, the intestinal release of modified F
MO
L microcapsules was 86 times of free F
MO
L. Therefore, this study confirmed that F
MO
L extracted with ChCl/urea has rich bioactive components, and microencapsulated F
MO
L has potential application in food industry.
Graphical abstract
Supplementary Information
The online version contains supplementary material available at 10.1007/s13399-023-03877-8.
A 5kw gasoline generator was converted into gas generator that could run on pipeline natural gas. A fire-restrain device and pressure reducer are designed to ensure safety. The measures such as increasing the compression ratio, enlarging spark advance and using a high-energy ignition are taken to improve the performance of the engine running on gas. Experiment has been done to compare the performances of the generator running on gasoline or natural gas. The result shows that the emission and economy of the generator are better when it runs on natural gas than that on gasoline.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.