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Antibiotics are widely used for animal production. However, the abuse of feeding antibiotics has resulted in drug resistance of pathogens, resulting in an imbalance of the intestinal flora of livestock and poultry (Chang, Wang, Regev-Yochay, Lipsitch, & Hanage, 2015) leaving antibiotic residues in livestock products and causing environmental pollution (Martinez, 2009). Therefore, researchers attempt to seek better antibiotic alternatives for laying hens. Antimicrobial peptides (AMP) are gene-encoded, ribosomal synthesized polypeptides, which usually have the common functions: anti-bacteria, anti-fungi and anti-virus (Hassan, Kjos, Nes, Diep,

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