This study explores the revenue and operation cost structures of private kindergartens in China as profitability is key to their sustainable development. Altogether 233 private kindergartens were sampled and involved in the survey study, which provides a quantitative analysis of the researcher-designed Questionnaire on the Revenue and Cost of Private Kindergartens (QRCPK). And 23 of these participating kindergartens were involved in a semi-structured interview. Analyses of both survey and interview data jointly indicated that: (1) the majority of the revenue of private kindergartens depended on the tuition fees; (2) their labor cost and rent costs are massive high; (3) the low profitability has been proved by the high cost-revenue ratio; (4) the poor cost accounting and financial management strategies hindered the sustainable development of private kindergartens.

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Pediatric acute respiratory distress syndrome (PARDS) is a severe form of respiratory failure associated with high mortality among children. The objective of this study is reported to explore the clinical function and molecular roles of microRNA-101-3p (miR-101-3p) in PARDS. The levels of miR-101-3p and mRNA levels of SRY-related high-mobility group box 9 (Sox9) were measured by quantitative reverse transcription polymerase chain reaction (RT-qPCR). Additionally, the diagnostic role of miR-101-3p was identified by using the Receiver operating characteristic (ROC) curve. The cell proliferation and apoptosis were examined through Cell Counting Kit-8 (CCK-8) assay and flow cytometry. To detect inflammation in cells, enzyme-linked immunosorbent assays (ELISA) were performed. The target gene of miR-101-3p was confirmed through data obtained from the luciferase activity. In patients with PARDS, miR-101-3p expression was decreased. Moderate and severe PARDS patients had lower levels of miR-101-3p than mild PARDS patients. The inflammatory progression was related to the aberrant expression of miR-101-3p in all PARDS children. MiR-101-3p was highly predictive for the detection of children with PARDS. In addition, miR-101-3p might protect A549 cells from abnormal proliferation, apoptosis, and inflammation caused by lipopolysaccharide (LPS). Sox9 might be a target gene of miR-101-3p and increased mRNA expression of Sox9 in LPS-treated A549 cells was inhibited by overexpression of miR-101-3p. Ultimately, this study suggested that reduced expression of miR-101-3p plays a role in PARDS, providing a novel angle to study the disease.