The aim of the present study was to develop and provide validation statistics for the Persian Injury-Psychological Readiness to Return to Sport scale (I-PRRS) following a cross-sectional and prospective cohort study design. The I-PRRS was forward/back-translated and culturally adapted into Persian language. The Persian I-PRRS was administered to 100 injured athletes (93 male; age 26.0 ± 5.6 years; time since injury 4.84 ± 6.4 months) and 50 healthy athletes (36 male; mean age 25.7 ± 6.0 years). The Persian I-PRRS was re-administered to 50 injured athletes at 1 week to examine test-retest reliability. There were no floor or ceiling effects confirming the content validity of Persian I-PRRS. The internal consistency reliability was good. Excellent test-retest reliability and agreement were demonstrated. The statistically significant difference in Persian I-PRRS total scores between the injured athletes and healthy athletes provides an evidence of discriminative validity. The Persian I-PRRS total scores were positively correlated with the Farsi Mood Scale (FARMS) total scores, showing construct validity. The principal component analysis indicated a two-factor solution consisting of "Confidence to play" and "Confidence in the injured body part and skill level". The Persian I-PRRS showed excellent reliability and validity and can be used to assess injured athletes' psychological readiness to return to sport among Persian-speaking populations.