No abstract

Some commercially available scientific products for ultrafiltration are readily available and have been used for a long time, especially in environmental sciences, life sciences and analysis of active ingredients from plants. Recent studies of metabolomics discuss the advantage and disadvantage of ultrafiltration versus protein precipitation and solid phase extraction. However, ultrafiltration techniques are rarely used in pharmaceutical analysis, even though they have advantages over solid phase extraction, such as easy to apply and no buffer preparations. The aim of this study is to develop a simple and innovative analysis technique for IBU determination from human plasma samples by ultrafiltration-based extraction in a pharmaceutical analysis. the prepared samples were analyzed using HPLC system equipped with C18 Column (100 x 4.6 mm, 3.5 µm). The flow rate was set to 1.0 mL min -1 while using isocratic elution with the mobile phase of [acetonitrile: phosphate buffer (pH 3.0, 15 mM) (56:44 v/v)] solution. Injection volume was 20 μL and UV detection was performed at 220 nm wavelenght. The developed method was validated according to FDA Bioanalytical Guidelines and found accurate, precise, sensitive, selective and efficient with the high recovery values samples.