from 2009 to 2014 years to study the effect of four factors affected callus induction of ovary cultur i.e.(cold pretreatment, chemical pretreatments, growth regulators and spikes sources. and establishment a technique to produce haploid plants. Three wheat varieties were used, Sids 1, Gemmeiza 9 and Giza 168. The results indicated that, chemical pretreatments by sterilization substances (Ethanol alcohol 70% or Mercurec chloride 1g/L or Thiourea 2g/L) were very important and essential to induce calli from ovaries. The combination between (1 and 2 mg /L 2,4-D) and (0 , 0.1 and 0.2 mg/L kinetien) gave the highest response of callus induction and plant regeneration percentages. The estimates were increased and developed with increasing the cultured area which the spiks were collected from it. The estimates were varied with varieties.