Background: Orexin plays a central role in the integration of sleep/wake states and feeding behaviors. Results: Orexin neurons were induced from pluripotent stem cells by supplementation of ManNAc. Conclusion: ManNAc induced switching of epigenetic factors from Sirt1/Ogt to Mgea5 at Hcrt gene locus. Significance: This study will be useful to investigate molecular mechanism in the orexin system and development of regenerative medicine.
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