Tea is one kind of plant that is popular as a beverage. These plants contain antioxidant potential of flavonoids derived from polyphenol compounds. The research aimed to examine the antioxidant activity and to determine the con-tent of total phenols and total flavonoids from ten grades of Indonesian black tea liqour. Antioxidant activity was measured by the method of free radical scavenging by DPPH, total phenol content measured by Follin-Ciocalteu method, and total flavonoid content was measured by the method of AlCl3. The results of this research showed that The highest total phenol content contained in Dust III with 225,80 mgGAE/100 g and the lowest content is BTL with 111,26 mgGAE/100 g. The test results showed that BBL has the highest total flavonoid content with 0,151 mg/g, while the lowest is BTL flavonoid with 0,086 mg/g. Meanwhile the highest antioxidant activity of ten grade of Indonesian black tea infusion obtained by Dust I, with IC50 97,00 μg/ml. Meanwhile the lowest antioxidant activity with IC50 178,56 μg/ml was obtained by BTL.

Green tea has bioactive component, it is polyphenol which has strong antioxidant. As the largest component of polyphenol, flavonoid is very effective as an antioxidant. The purpose of this study was to determine antioxidant activity, phenol total and flavonoid total in green tea from three different estates, they were Cikajang Kab. Garut, Taraju Kab. Tasikmalaya, and Ciwidey Kab. Bandung. The results of this study show that seven samples have good antioxidant activity, but green tea which came from Cikajang (P-IRT No. 810320501698) has the highest IC50 grade it was 21,44 μg/ml. Beside that samples from Cikajang (P-IRT No. 810320501698) has highest total phenol content it was 334,68± SD 0,89/100 g samples and also has the highest total flavonoid it was 0,34 mg Kuersetin/g samples ± SD 0,21. On the contrary, green tea which has the lowest IC50 grade came from Taraju, the grade was 28,03 μg/ml, the samples has the lowest total phenol content it was 208,91± SD 4,47 mgGAE/100 g, and also has the lowest total flavonoid it was 0,207 mg Kuersetin/g samples ± SD 0,00.

The World Health Organization (WHO) states, in 2015, there are an estimated 9 million people who die of cancer and in 2030 there are an estimated 11.4 million deaths from cancer. Blood cancer reaches 3% of all cancers found in humans and is most common in children. The cancer drug that is available today has quite severe side effects, therefore in this study an antileukemia activity test will be carried out on water, 70% ethanol, and n-hexane extract of dates (Phoenix dactylifera). This study, the extracts from dates will be tested for cytotoxicity on Murine Leukemia P388 cells using the MTT Assay method. The IC50 value of extract of dates is used as an assessment of the activity of palm fruit extract as antileukemia. Citotoxicity assay against Murine Leukemia P388 cells by MTT assay, showed IC50 value is more than 100 µg/mL for all extracts. This result informed that the water, ethanol, and n-hexane extract of dates fruit are less potencial as a antileukemia agent.

Plants of Piperaceae family have been widely known for its antiinfl ammation, anticancer, and other activities related to immune system. Several plants of Piperaceae family have also been reported to have activity as immumomodulators . Piper cubeba, Piper aduncum, and Piper retrofractum belong to piperaceae family and widely distributed in West Java, Indonesia. However, research related to these plants are still limited. Thereby, purpose of this study were to evaluate the immunomodulatory activity of ethanolic extracts of these plants in Balb/C mice. Animals were divided into 12 groups : normal control group, immunosuppressant comparison, immunostimulant comparison, and low, moderate, and high dose of P.cubeba, P.aduncum, and P.retrofractum. Phagocytosis index, peripheral blood leukocyte levels, and splenic leukocyte levels were analyzed. The results showed that ethanolic extract of P.cubeba has immunosuppressant activity, meanwhile P.retrofractum tend to have immunostimulant activity. Low doses of P.aduncum acts as immunosuppressant, and at medium and high doses has a tendency to be immunostimulant. Ethanolic extract of P.cubeba, P.aduncum, and P.retrofractum aff ect the number of peripheral blood granulocyte components, but did not aff ect the number of splenic granulocyte components. These fi ndings conclude that piperaceae family, especially P.cubeba, P.aduncum, and P.retrofractum, are potential source to be developed as immunomodulators.Abstrak: Tanaman dari keluarga Piperaceae telah diketahui memiliki aktivitas sebagai antiinfl amasi, antibakteri, antikanker, dan aktivitas lain yang mengarah pada aktivitas sistem imun. Beberapa tanaman dari keluarga ini juga telah dilaporkan memiliki aktivitas sebagai immunomodulator. Kemukus (Piper cubeba), kiseureuh (Piper aduncum), dan cabe jawa (Piper retrofractum) adalah tiga tanaman dari keluarga piperaceae yang banyak tersebar di daerah Jawa Barat, namun belum banyak diteliti. Penelitian bertujuan untuk mengevaluasi aktivitas imunomodulator ketiga tanaman dari keluarga piperaceae tersebut pada mencit jantan galur Balb/c dengan metode bersihan karbon. Penelitian dilakukan dengan membagi hewan coba menjadi 12 kelompok, yaitu kelompok kontrol normal, pembanding imunosupresan, pembanding imunostimulan, serta masing-masing tiga kelompok variasi dosis kemukus, kiseureuh, dan cabe jawa. Parameter aktivitas imunomodulator ditinjau dari indeks fagositosis, kadar leukosit darah tepi, dan kadar leukosit limpa. Hasil penelitian menunjukkan ekstrak etanol kemukus (P. cubeba) memiliki aktivitas imunomodulator sebagai imunosupresan, sedangkan cabe jawa (P. retrofractum) sebagai imunostimulan. Ekstrak etanol kiseureh (P. aduncum) dosis rendah memiliki aktivitas imunosupresan, sedangkan pada dosis sedang dan tinggi memiliki kecenderungan sebagai imunostimulan. Ketiga tanaman tersebut juga mempengaruhi jumlah komponen granulosit darah tepi, namun tidak mempengaruhi jumlah komponen granulosit limpa. Temuan ini menunjukkan bahwa P.cubeba, P.aduncum, and P.retrofractum berpotensi untuk d...